For the 2016 Spring League, 46 high school teams are assigned conferences based on a similar number of team members.
(#) = Number of athlete’s scores used for each team to determine True Team® Scoring points earned.
| CLASS A | True Team # |
| CONFERENCE 1 | 5 |
| Randolph High School | |
| Green Bay Preble High School | |
| Portage High School | |
| River Ridge High School | |
| CONFERENCE 2 | 8 |
| Clintonville High School | |
| Mishicot High School | |
| Turtle Lake High School | |
| Washburn High School | |
| Sauk Prairie High School | |
| Wolf River Lutheran High School | |
| Royall High School | |
| Superior High School | |
| CONFERENCE 3 | 11 |
| Drummond High School | |
| Shawano Community High School | |
| Clayton High School | |
| Potosi High School | |
| Rio High School | |
| Mineral Point Unified High School | |
| Nekoosa High School | |
| CONFERENCE 4 | 13 |
| Seneca High School | |
| Elmwood High School | |
| Northwestern High School | |
| Cassville High School | |
| Lancaster High School | |
| De Soto High School | |
| Cochrane-Fountain City High School | |
| CONFERENCE 5 | 17 |
| Central/Logan High Schools | |
| Colfax High School | |
| Fox Valley Lutheran High School | |
| Siren High School | |
| Cumberland High School | |
| Denmark High School | |
| Unity High School | |
| Dodgeville High School | |
| Durand High School | |
| CONFERENCE 6 | 24 |
| Grantsburg High School | |
| Gale-Ettrick-Trempealeau High School | |
| Prairie du Chien High School | |
| Clear Lake High School | |
| Frederic High School | |
| St. Croix Central High School | |
| CONFERENCE 7 | 32 |
| Ashland High School | |
| Ellsworth Senior High School | |
| St. Croix Falls High School | |
| Boscobel High School | |
| Tomah High School |