For the 2019 Spring League, 87 high school teams are assigned to 11 conferences based on a similar number of team members.
(#) = Number of athlete’s scores used for each team to determine True Team™ scoring points earned.
| CONFERENCE 1 (6) |
| Brookfield Central High School |
| Chilton High School |
| Faith Christian School |
| La Farge High School |
| Loyal High School |
| New Lisbon High School |
| Stockbridge High School |
| Washington Island High School |
| Wolf River Lutheran High School |
| CONFERENCE 2 (8) |
| Colby High School |
| Fall River High School |
| Iola-Scandinavia High School |
| Marinette High School |
| Mishicot High School |
| Sauk Prairie High School |
| Seneca High School |
| Valders High School |
| CONFERENCE 3 (11) |
| Cassville High School |
| Clintonville High School |
| Drummond High School |
| North Crawford High School |
| Plymouth High School |
| Randolph High School |
| Waterloo High School |
| CONFERENCE 4 (13) |
| Alma High School |
| Elkhart Lake-Glenbeulah High School |
| Elmwood High School |
| Siren High School |
| St. Croix Central High School |
| Turtle Lake High School |
| Weston High School |
| CONFERENCE 5 (15) |
| Ashland High School |
| Benton High School |
| Cambria-Friesland High School |
| Gillett High School |
| Hillsboro High School |
| Kickapoo High School |
| Lancaster High School |
| Potosi High School |
| River Ridge High School |
| CONFERENCE 6 (17) |
| Denmark High School |
| Mineral Point Unified High School |
| New Holstein High School |
| Northwestern High School |
| Prairie du Chien High School |
| Superior High School |
| Washburn High School |
| Wauzeka-Steuben High School |
| CONFERENCE 7 (20) |
| Aquinas High School |
| Clayton High School |
| Cumberland High School |
| Howards Grove High School |
| Notre Dame Academy |
| Portage High School |
| Royall High School |
| Shawano Community High School |
| Southwestern High School |
| CONFERENCE 8 (22) |
| Cochrane-Fountain City High School |
| Grantsburg High School |
| Neillsville High School |
| Preble High School |
| Rio-Pardeeville High School |
| Shullsburg High School |
| CONFERENCE 9 (25) |
| Bangor High School |
| Bonduel High School |
| Frederic High School |
| Gale-Ettrick-Trempealeau High School |
| Lincoln-Nekoosa-Port Edwards-Adams/Friendship High |
| Melrose-Mindoro High School |
| Mondovi High School |
| Reedsburg Area High School |
| Riverdale High School |
| CONFERENCE 10 (28) |
| Boscobel High School |
| Clear Lake High School |
| De Soto High School |
| Durand High School |
| Ellsworth Senior High School |
| Tomah High School |
| Unity High School |
| West Salem High School |
| CONFERENCE 11 (38) |
| Central/Logan High Schools |
| Dodgeville High School |
| Fox Valley Lutheran High School |
| Holmen High School |
| Luxemburg-Casco High School |
| River Valley High School |
| St. Croix Falls High School |